Table 2 Microsatellite polymorphic markers used for GI analysis

NC2β 5'

D1S2776

92982656 – 92982869

0.75

5' AATGCCTGTCTTTATCCCTG 3'

5' AATGTAAGAGAAATGCCCCT 3'

196 – 212

95°C 10 min (95°C 30 sec, 52°C 75 sec, 72°C 30 sec) 30×; 72°C 10 min

NC2β 3'

D1S2813

95036107 – 95036299

0.72

5' CTTTTGACTCACTGGAAGACAT 3'

5' CCCCACCGTATCTGGTAT 3'

185 – 205

95°C 10 min (95°C 30 sec, 55°C 75 sec, 72°C 30 sec) 30×; 72°C 10 min

NC2β 3'

D1S2664

95718483 – 95718723

0.73

5' CAGCCCACAGAATAACACTG 3'

5' TTCATGCTATGATTTTCCGC 3'

202 – 254

95°C 10 min (95°C 30 sec, 54°C 75 sec, 72°C 30 sec) 30×; 72°C 10 min

TAF12 5'

D1S2787

27999563 – 27999728

0.76

5' TTTAACCCTGGAAGGTTGAG 3'

5' ACAGGACAATGCTGTCAGTATG 3'

137 – 167

95°C 10 min (95°C 30 sec, 52°C 75 sec, 72°C 30 sec) 30×; 72°C 10 min

TAF12 3'

G60315

30205319 – 30205625

-

5' AGCTGAGTCAGGGAAACCCATT 3'

5' TGTGCTCTTCAATGTGTTAGGGA 3'

307 – 340

95°C 10 min (95°C 30 sec, 56°C 75 sec, 72°C 30 sec) 30×; 72°C 10 min

TAF13 5'

D1S2778

108849441 – 108849609

0.66

5' CACAGTTAAATTGCATTTCC 3'

5' GCTCACCATAAACAAGAGG 3'

161 – 173

95°C 10 min (95°C 30 sec, 50°C 75 sec, 72°C 30 sec) 30×; 72°C 10 min

TAF13 3'

D1S221

110103235 – 110103467

0.75

5' CCTACAACTCCATCCTGTCC 3'

5' GTCTTAAGTCGCTCTGCCTG 3'

215 – 225

95°C 10 min (95°C 30 sec, 56°C 75 sec, 72°C 30 sec) 30×; 72°C 10 min

NC2α 3'

D11S1889

67069719 – 67069901

0.67

5' AGCTGGACTCTCACAGAATG 3'

5'CAAGAGGCTGGTAGAAGGTG3'

183 – 207

95°C 10 min (95°C 30 sec, 60°C 30 sec, 72°C 30 sec) 30×; 72°C 10 min

NC2α 3'

D11S4178

67945684 – 67945935

0.69

5' CAGGCCCAGTCTCTTG 3'

5' CGTGTCCAGATGAAAGTG 3'

237 – 260

95°C 10 min (95°C 30 sec, 52°C 75 sec, 72°C 30 sec) 30×; 72°C 10 min.

GTF2B 5'

D1S2856

82130207 – 82130465

0.5

5' AGCTCTGTGACATTGGATAA 3'

5' CAGAACATAATAAGTGTGGCTA 3'

257 – 263

95°C 10 min (95°C 30 sec, 53°C 75 sec, 72°C 30 sec) 30×; 72°C 10 min.

GTF2B 5'

D1S454

82540746 – 82540872

0.56

5' TGTTAGTTCCTGTTCTTGGTGA 3'

5' TTCCCTGGAAACAACCATAA 3'

155 – 163

95°C 10 min (95°C 30 sec, 58°C 75 sec, 72°C 30 sec) 30×; 72°C 10 min

GTF2B 3'

D1S435

91331437 – 91331556

0.73

5' GGCCACATGGGAATTTTCT 3'

5' AGCAGTTCAAGGCCACAGT 3'

157 – 177

95°C 10 min (95°C 30 sec, 55°C 75 sec, 72°C 30 sec) 30×; 72°C 10 min