Figure 3

Immunofluorescence detection of MT1/2 in HPR-1, BPH, and PC-3 cells. The immunofluorescence statining of MT1/2 was examined in the cells, HPR-1 (left), BPH (middle), and PC-3 (right). The background of non-specific staining was monitored using the red FLURO-conjugated secondary antibody only indicated as the control (top row), in which the cells were also stained with Hoechost 33258 shown the blue-stained nuclei to remark the cells. The differential immunoreactivities of the cells to MT1/2 antibody were determined by the intensity of red fluorescence between the cell lines (middle row). The images with both MT1/2 and Hoechost nuclear staining (bottom row) were the attempts to detect the cellular distribution of MT1/2. The pinkish colour resulted from the merged blue and red colour was observed in some nuclei of HPR-1 cells. All images were observed and recorded under the same settings of a fluorescence microscope with the magnification of × 600.