Figure 7From: Differential expression of metallothioneins (MTs) 1, 2, and 3 in response to zinc treatment in human prostate normal and malignant cells and tissuesDetection of zinc regulation of MT 3 expression in BPH cells by Western blot analysis. BPH cells were treated with zinc (15 μM) or without zinc (control) for 3, 6, 12, and 24 h, respectively. The protein extracts (50 μg) of samples from each time point were separated by SDS-PAGE, and the MT 3 was detected by Western blot analysis (top panel) with rabbit anti-MT 3 antibody and β-actin was used as an internal control. The density of specific MT 3 signals was analyzed and data are shown on the lower panel. The experiment was repeated at least thrice.Back to article page