Figure 1

EPLIN-α expression in breast cancer tissues and cell lines. a: Immunohistochemical staining of EPLIN-α in normal mammary tissues (left panel) and tumour tissues (right panel) from different patients. The tumour tissues were from patients with different tumour grade. D was a grade-1 tumour (Patient ID 123), E a grade-2 tumour (ID 75a) and F grade-3 tumour (ID 113). Magnification was ×200 for the micrographs. b1: Detection of the EPLIN-α transcript using RT-PCR in a panel of human breast, endothelial and stromal fibroblast cell lines. b2: Detection of the EPLIN-α transcript in a panel of matched normal and tumour tissues. Actin was used as the housekeeping control. c: quantitative analysis of the EPLIN-α transcript in normal and tumour tissues. d: Levels of expression of EPLIN-α transcripts in breast tumour tissues in connection with TNM status (left; * p = 0.0029 vs TNM1 tumours). e: Levels of expression of EPLIN-α in breast tumour tissues in connection with grade (left, * p < 0.05 vs grade 1 tumours) and F: with nodal status (right). Inserts in d, e and f are values of EPLIN-α transcript that have been normalised by CK19 (shown as EPLIN:CK19 ratio).