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Figure 2 | Molecular Cancer

Figure 2

From: Rac1 GTPase and the Rac1 exchange factor Tiam1 associate with Wnt-responsive promoters to enhance beta-catenin/TCF-dependent transcription in colorectal cancer cells

Figure 2

Rac1 enhances the transcriptional activation of Wnt target genes in HCT116 cells. (A) HCT116 cells with stable inducible expression of V12Rac1 were serum starved (0.1% FBS) and treated with (+) or without (-) 0.1 μg/ml dox for 24 hours. Total RNA was analyzed by quantitative RT-PCR for the detection of Rac1 and c-Myc mRNA. (B) HCT116 cells were transiently transfected with either non-silencing (N.S.) siRNA, Rac1-specific siRNA, or GAPDH siRNA for 48 hours. Total RNA was analyzed by quantitative RT-PCR for the detection of Rac1 and GAPDH transcript levels (top) or c-Myc transcript levels (bottom). For quantitative RT-PCR experiments, transcript levels of Rac1, c-Myc, and GAPDH were normalized to transcript levels of β-actin. Bars represent mean ± S.E., *p < 0.05, **p < 0.005.

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