Figure 1
From: A quantitative PCR method to detect blood microRNAs associated with tumorigenesis in transgenic mice
High yields of intact RNA from stabilized mouse blood and use in qRT-PCR-based microRNA profiling. (A) Yield of RNA isolated using the Ambion® Mouse RiboPure™-Blood RNA Isolation kit. Blood samples were obtained by cardiac puncture or via tail vein bleed into a 2 mL tube preloaded with RNAlater® Solution. RNA was isolated, quantified, and examined on an Agilent® 2100 bioanalyzer (Agilent Technologies). Cardiac puncture samples were diluted 1:10 before running. Representative bioanalyzer scans for (B) Quality of cardiac puncture blood RNA and (C) Quality of tail vein blood RNA are shown with major features marked. The lack of significant species between the peaks indicates that RNA is highly intact. (D) Comparison of SYBR®-based and TaqMan®-based miRNA quantitation. A panel of 26 miRNA were quantitated in blood from normal control mice using both SYBR qRT-PCR assays and TaqMan-based qRT-PCR assays. Correlation between results from the two methods was 0.86.