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Table 1 The primer sequences and annealing temperatures for methylation analysis

From: Similarity of aberrant DNA methylation in Barrett's esophagus and esophageal adenocarcinoma

Gene Forward primer Reverse primer Annealing temperature (°C)
APC GAAGYGGAGAGAGAAGTAGTTG ACRAACTACACCAATACAACCACATA 55
CDKN2A TYGGYGGYGGGGAGTAGTATGGAGTTT RTTAAACAACRCCCCCRCCTCCAACAA 60
ID4 GGGGYGTAYGGTTTTATAAATATAGTTG TAATCACTCCCTTCRAAACTCCGACTAAA 55
MGMT 5G5GTTT5GGATATGTTGGGATAGTT AC5AAAC5ACCCAAACACTCACCAAA 55
RBP1 TGTGYGYGTTGGGAATTTAGTTG CRAAAAATAACTAAAACCAATTAACCACAAA 55
RUNX3 YGTYGTTTTTTGYGTTTTGAGGTT ACTTAAATCTACRAAAATACRCATAACAA 55
SFRP1 ATTTTYGGGAGTYGGGGYGTATT RACCAATAACRACCCTCRACCTA 57
TIMP3 TTTGAGGGGGYGGGTTTTAATAGTT AACRACCTCCCRACGAAAAAACAAA 55
TMEFF2 TTGTTTTTTYGTYGGGTGTTATTGTTAT AACAAACRACTTCCRAAAAACACAAA 55