Similarity of aberrant DNA methylation in Barrett's esophagus and esophageal adenocarcinoma

Table 1 The primer sequences and annealing temperatures for methylation analysis

Gene	Forward primer	Reverse primer	Annealing temperature (°C)
APC	GAAGYGGAGAGAGAAGTAGTTG	ACRAACTACACCAATACAACCACATA	55
CDKN2A	TYGGYGGYGGGGAGTAGTATGGAGTTT	RTTAAACAACRCCCCCRCCTCCAACAA	60
ID4	GGGGYGTAYGGTTTTATAAATATAGTTG	TAATCACTCCCTTCRAAACTCCGACTAAA	55
MGMT	5G5GTTT5GGATATGTTGGGATAGTT	AC5AAAC5ACCCAAACACTCACCAAA	55
RBP1	TGTGYGYGTTGGGAATTTAGTTG	CRAAAAATAACTAAAACCAATTAACCACAAA	55
RUNX3	YGTYGTTTTTTGYGTTTTGAGGTT	ACTTAAATCTACRAAAATACRCATAACAA	55
SFRP1	ATTTTYGGGAGTYGGGGYGTATT	RACCAATAACRACCCTCRACCTA	57
TIMP3	TTTGAGGGGGYGGGTTTTAATAGTT	AACRACCTCCCRACGAAAAAACAAA	55
TMEFF2	TTGTTTTTTYGTYGGGTGTTATTGTTAT	AACAAACRACTTCCRAAAAACACAAA	55

ISSN: 1476-4598