Table 3 The primer sequences and annealing temperatures for qRT-PCR
From: Similarity of aberrant DNA methylation in Barrett's esophagus and esophageal adenocarcinoma
Gene | Forward primer | Reverse primer | Annealing temperature (°C) |
|---|---|---|---|
APC | TGGAGAACTCAAATCTTCGACA | CAATCTGTCCAGAAGAAGCCATA | 62 |
CDKN2A | GAGGCCGATCCAGGTCAT | CCAGCGTGTCCAGGAAG | 62 |
HMBS | ACATGCCCTGGAGAAGAATG | TTGGGTGAAAGACAACAGCA | 57 |
ID4 | CCGAGCCAGGAGCACTAGAG | CTTGGAATGACGAATGAAAACG | 60 |
MGMT | TGGAGCTGTCTGGTTGTGAG | GCTGGTGGAAATAGGCATTC | 60 |
RBP1 | AGGCATAGATGACCGCAAG | CTCATCACCCTCGATCCAC | 62 |
RUNX3 | GCAGGCAATGACGAGAACTA | CAGTGATGGTCAGGGTGAAA | 57 |
SFRP1 | TTGAGGAGAGCACCCTAGGC | TGTGTATCTGCTGGCAACAGG | 60 |
TIMP3 | CCAGGACGCCTTCTGCAAC | CCTCCTTTACCAGCTTCTTCCC | 60 |
TMEFF2 | CAATGGGGAGAGCTACCAGA | TGGACTCCATCTCCAGATCC | 62 |