Skip to main content
Figure 1 | Molecular Cancer

Figure 1

From: Human ESCs predisposition to karyotypic instability: Is a matter of culture adaptation or differential vulnerability among hESC lines due to inherent properties?

Figure 1

Molecular cytogenetic analysis of HS181 hESC line. HS181 hESC line was genetically stable for 71 passages in feeders. Upon being transferred to feeder-free conditions and split using enzymatic methods, the HS181 hESC line gained a chromosome 12 as early as passage 17 and an additional marker, shown by SKY to be an extra chromosome 20 by passage 21. The level of mosaicism gradually increased from 26% (p17) to 89% (p30). In conventional karyotyping analysis (A) and CGH (C), the blue arrows indicate chromosomal gains. In the SKY images (B), the white arrows confirm the gain of chromosome 12 and characterized the marker seen by G-banding as a partial gain of chromosome 20.

Back to article page