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Figure 3 | Molecular Cancer

Figure 3

From: Human ESCs predisposition to karyotypic instability: Is a matter of culture adaptation or differential vulnerability among hESC lines due to inherent properties?

Figure 3

Molecular cytogenetic analysis of SHEF-1 hESC line. In contrast to HS181 and SHEF-3 hESC lines, the SHEF-1 hESC line was genetically stable after as many as 185 passages in feeders. Upon being transferred to feeder-free conditions, the SHEF-1 hESC line displayed no karyotypic changes assessed by G-banding (A), SKY (B) and CGH (C) after further 30 passages in feeder-free conditions despite being split by means of enzymatic methods throughout over 215 passages (almost 4 years in in vitro culture). This data suggests that although culture conditions may partially contribute to chromosome stability, some hESC lines are inherently more predisposed than others to karyotypic changes.

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Molecular Cancer

ISSN: 1476-4598