Figure 3From: A conserved acidic patch in the Myb domain is required for activation of an endogenous target gene and for chromatin bindingMutations in the third repeat bind DNA when the salt bridge is intact. A. Electrophoretic mobility shift assays (EMSA) using nuclear lysates containing c-Myb VP16 (c-MybVP) wild-type and mutant fusion proteins were performed. c-MybVP lysates are labeled: – = no lysate; v/o = lysate with vector only (no c-MybVP protein); wt = wild-type; mR3-1,-2, and -3 = third repeat mutants. B. Western blot analysis of nuclear lysates used in the EMSA. The 5E Myb-specific monoclonal antibody reveals that similar wild-type and mutant proteins are present in the assay. c-MybVP lysates are labeled as in A. Bars represent the 7B SDS PAGE molecular weight markers (Sigma-Aldrich) with bands from top to bottom of 180, 116, 84, 58, 48.5, 36.5 kDa.Back to article page