Table 1 Summary of c-Myb mutants.
| Â | Â | Â | Â | Â | Â | % Reporter Activity | Â | ||
|---|---|---|---|---|---|---|---|---|---|
|  | Repeat-substitutions | Missing salt bridges | DNA-binding | Nuclear | Stability | c-Myb | c-MybVP | c-MybΔ | Endogenous target gene activation |
Wt | Â | 0 | +++ | ++ | + | 100 | 100 | 100 | ++ |
R1 | 1-1 AEDA | 0 | +++ | ++ | + | 220 | 120 | 168 | nd |
R1 | 1–2 AEAA | 1 | +++ | ++ | + | 45 | 100 | 120 | + |
R1 | 1–3 AADA | 1 | +++ | ++ | + | 98 | 77 | 83 | + |
R1 | 1–4 AAAA | 2 | + | ++ | + | 10 | 20 | 47 | +/- |
R2 | 2-1 AED | 0 | +++ | ++ | + | 94 | 74 | nd | nd |
R2 | 2-2 AEA | 1 | -- | +/- | + | > 1 | > 1 | nd | nd |
R2 | 2–3 AAA | 2 | -- | - | +/- | > 1 | > 1 | nd | - |
R3 | 3-1 EAEA | 0 | +++ | ++ | + | 25 | 100 | 33 | - |
R3 | 3-2 EAAA | 1 | -- | ++ | +/- | > 1 | > 1 | nd | - |
R3 | 3-3 AAEA | 0 | +++ | ++ | + | 10 | 98 | 19 | - |