Figure 7
2-D 1H-13C HSQC analysis of Rh30 cells and myocytes. Both cells were cultured in [U-13C]-glucose for 24 hr before trichloroacetic acid extraction and HSQC measurement at 14.4 T. The spectra were recorded with 0.14 s acquisition in t2 and 34 ms in t1. The data table in t1 was linear predicted to 1024 complex points and zero filled to 2048, so that 13C-13C couplings (35–45 Hz) were resolved. Panel C displays the 2-D contour map of the Rh30 cell extract while panels B and A are respectively the 1-D 13C projection spectra of the 2-D contour maps for Rh30 and myocytes. Both A and B were normalized to cell dry weight but A was plotted at 10× scale relative to B.