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Table 1 Quantification of labeled metabolites in the media by 1H NMR analysis.

From: Rhabdomyosarcoma cells show an energy producing anabolic metabolic phenotype compared with primary myocytes

Cell [13C Lac] mM %13CLac [13C Ala] mM %13CAla [Thr] mM [Glc] mM Δ[Glc]a mM %Glc->Lacb
Rh30 (24 h) 4.8 ± 0.6 83 ± 1 0.35 ± .03 67 ± 4 0.15 6.1 ± 0.6 4.6 ± .6 52 ± 7.5
Myocyte(48 h) 1.2 ± 0.2 64 ± 2 <0.01 <5 0.15 4.8 ± .5 5.9 ± .5 10 ± 1.4
  1. Concentrations and 13C enrichments of selected metabolites in the medium were determined by NMR integrations as described in the Methods.
  2. a change in medium glucose concentration (= consumption).
  3. b percentage of glucose consumed converted to secreted lactate (cf. text).