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Table 1 Quantification of labeled metabolites in the media by 1H NMR analysis.

From: Rhabdomyosarcoma cells show an energy producing anabolic metabolic phenotype compared with primary myocytes

Cell

[13C Lac] mM

%13CLac

[13C Ala] mM

%13CAla

[Thr] mM

[Glc] mM

Δ[Glc]a mM

%Glc->Lacb

Rh30 (24 h)

4.8 ± 0.6

83 ± 1

0.35 ± .03

67 ± 4

0.15

6.1 ± 0.6

4.6 ± .6

52 ± 7.5

Myocyte(48 h)

1.2 ± 0.2

64 ± 2

<0.01

<5

0.15

4.8 ± .5

5.9 ± .5

10 ± 1.4

  1. Concentrations and 13C enrichments of selected metabolites in the medium were determined by NMR integrations as described in the Methods.
  2. a change in medium glucose concentration (= consumption).
  3. b percentage of glucose consumed converted to secreted lactate (cf. text).
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Molecular Cancer

ISSN: 1476-4598