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Table 3 Quantification of 13C enrichment in cellular metabolites by 1H NMR analysis: Pentose phosphate and pyrimidine biosynthesis

From: Rhabdomyosarcoma cells show an energy producing anabolic metabolic phenotype compared with primary myocytes

  Ribose ring   Base ring
Condition %13C Pur %13C Pyr [%13C Uri
Rh30 (FCS) 95 ± 2 80 ± 2 50 ± 3(26 ± 2)a
Rh30 (BSA) 82 ± 2 88 ± 2 45 ± 2(21 ± 2)a
Myocytes >90 <5 <5
  1. a All 4 possible isotopomers at the C5, C6 positions were observed. first entry: percentage of all forms of 13C5 and 13C6 of U. The value in parenthesis the percentage of doubly labeled C5 and C6.