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Table 3 Quantification of 13C enrichment in cellular metabolites by 1H NMR analysis: Pentose phosphate and pyrimidine biosynthesis

From: Rhabdomyosarcoma cells show an energy producing anabolic metabolic phenotype compared with primary myocytes

 

Ribose ring

 

Base ring

Condition

%13C Pur

%13C Pyr

[%13C Uri

Rh30 (FCS)

95 ± 2

80 ± 2

50 ± 3(26 ± 2)a

Rh30 (BSA)

82 ± 2

88 ± 2

45 ± 2(21 ± 2)a

Myocytes

>90

<5

<5

  1. a All 4 possible isotopomers at the C5, C6 positions were observed. first entry: percentage of all forms of 13C5 and 13C6 of U. The value in parenthesis the percentage of doubly labeled C5 and C6.
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Molecular Cancer

ISSN: 1476-4598