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Figure 2 | Molecular Cancer

Figure 2

From: Bone marrow ectopic expression of a non-coding RNA in childhood T-cell acute lymphoblastic leukemia with a novel t(2;11)(q11.2;p15.1) translocation

Figure 2

Results of the HPS5 and DA926692 gene expression analysis: A) and B) RQ-PCR analyses of HPS5, and DA926692 exon1, respectively, in the present case [t(2;11), four control childhood T-ALL samples [immunophenotype: early (#1,2), thymic (#3), and mature (#4) T-ALL], the mean Ct value of the controls (mean), normal BM and PB. A) The results showed no statistically significant change in HPS5 expression level with primers for exon1 (HPS5ex1-19F+HPS5ex1-96R) and exons 20-21 (HPS5ex20-3216F and HPS5ex21-3255R), if compared with the mean Ct value of the childhood T-ALL controls. B) The results showed comparable DA926692 transcriptional levels, when evaluated with exon 1 primers (DA926692ex2F and DA926692ex2R), between the patient with t(2;11) and the mean Ct value of the controls. C) RT-PCR results obtained with DA926692ex2anewF and DA926692ex2anewR (Table 1), showing a band of 173 bp only in the patient's bone marrow (lane 1) and normal genomic DNA (lane 8). Lanes 2-5 correspond to four control childhood T-ALL bone marrow. Lanes 6, 7 and 9 are normal bone marrow, normal peripheral blood, and blank, respectively. M: 2-Log DNA Ladder (New England Biolabs, Milan, Italy). D) Control RT-PCR with ACTB primers to check the RNA quality, excluding contamination of genomic DNA in the patient's bone marrow RNA.

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Molecular Cancer

ISSN: 1476-4598