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Figure 3 | Molecular Cancer

Figure 3

From: Bone marrow ectopic expression of a non-coding RNA in childhood T-cell acute lymphoblastic leukemia with a novel t(2;11)(q11.2;p15.1) translocation

Figure 3

Tissue expression pattern of EST DA926692: DA926692 expression analysis was performed using cDNA multiple tissue (Ambion, Milan, Italy) First Choice Total RNA Survey Panel (Catalog No. AM6000) (adipose, bladder, brain, cervix, esophagus, heart, kidney, liver, lung, ovary, placenta, prostate, skeletal muscle, small intestine, spleen, testes, thymus, thyroid, trachea), according to the manufacturer's instructions. We have also tested a pool of tonsil cDNA extracted from three normal individuals. The primer combination used was DA926692ex2anew (F+R). The results, evaluated by RQ-PCR, showed positivity of spleen, ovary, placenta, thyroid, lung, and colon. Compared with the reference ACTB gene expression levels (data not shown), the overall DA926692 expression in the positive tissues was found to be relatively low (2-ΔCt ≤ 1.8 × 10-4). The strongest expression was seen in the colon.

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Molecular Cancer

ISSN: 1476-4598