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Figure 7 | Molecular Cancer

Figure 7

From: Promoter hypermethylation of the SFRP2 gene is a high-frequent alteration and tumor-specific epigenetic marker in human breast cancer

Figure 7

SFRP2 inhibits proliferation in breast epithelial cells. (A) MCF10A cells transfected with empty vector (Mock), SFRP2-expression vector, WNT1-expression vector or a combination of SFRP2+WNT1-expression vectors were subjected to XTT proliferation assays. At three subsequent time points, optical densities (OD) of the supernatants were measured. RNA Expression of the respective genes was surveyed in parallel 4 days post transfection. GAPDH served as cDNA loading control. SFRP2 was able to reduce a proliferative effect mediated by WNT1 overexpression, whereas the inhibiting effect of SFRP2 on cells not stimulated by WNT1 was only marginal (HA). (B) Long-term inhibiting effects of SFRP2 on the ability to form colonies in MCF10A cells. Cells were either transfected with empty vector (Mock) or with SFRP2-expression vector, and after 3 weeks of selective pressure (antibiotic G418) fixed, stained and photographed. Non-transfected wild type cells (WT) are shown as controls with (+) and without (-) the application of G418. The difference in colony numbers between mock-transfected and SFRP2-transfected cells is significant (P = 0.045; two-tailed Mann-Whitney U-test).

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