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Figure 2 | Molecular Cancer

Figure 2

From: ALDH isozymes downregulation affects cell growth, cell motility and gene expression in lung cancer cells

Figure 2

A. The effect of siRNA against ALDH1A1 and ALDH3A1 on ALDH activity and protein. The mean percent ± SD of remaining ALDH activity (in comparison to control GFP group) as measured by spectrophotometry enzyme activity assay is shown in A549 cells transduced by lentiviral vector containing siRNA against ALDH1A1 (Lenti 1), ALDH3A1 (Lenti 3), both vectors (Lenti 1+3), green fluorescent protein (GFP), and untreated parent cell line (WT). The results represent at least 3 different experiments. Furthermore, Western blot analysis shows the decrease in ALDH1A1 and ALDH3A1 proteins in Lenti 1+3 group in comparison to WT and GFP control groups. Actin is shown as a loading control. The results are typical of an experiment performed at least twice. B. Lentivirally mediated expression of siRNA constructs (shown in A.) results in similar effects on ALDH activity in H522 cell line.

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