Figure 3
The effect of siRNA expression on cell proliferation and motility. A. Knock-down of ALDH1A1, ALDH3A1 or both results in significant decrease in cell proliferation in comparison to WT and GFP controls from A549 and H522 cells (P < 0.024, except for A549 Lenti-1 with P value of 0.052). The horizontal line indicates the starting cell number (2 × 105/group) incubated, while the bars represent mean ± SD of viable cell counts after 72 hr incubation obtained from at least 2 experiments. Scratch wound migration assay results are shown. B. The bars represent mean ± SD of 4 measurements of wound healing in the 5 different cell lines of A549 cell. Significant decrease in cell migration into scratch wound is shown after 20 hr in cells with ALDH1A1 (Lenti-1), ALDH3A1 (Lenti-3) and both isozymes (Lenti 1+3) knock-down in comparison to WT cells or cells transduced with GFP siRNA (P < 0.005). C. An example of the delayed wound healing seen with the knock-down of both isozymes (Lenti 1+3 cells) on wound healing in comparison to WT and GFP cells.