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Figure 1 | Molecular Cancer

Figure 1

From: Membrane androgen receptor activation triggers down-regulation of PI-3K/Akt/NF-kappaB activity and induces apoptotic responses via Bad, FasL and caspase-3 in DU145 prostate cancer cells

Figure 1

Modulation of the dynamic equilibrium between G- and F- actin and induction of apoptosis in testosterone-BSA stimulated DU145 cells. A) 24 h serum starved cells were stimulated with 10-7 M testosterone-BSA or DHT for the indicated time points. F- and G- actin were measured by quantitative immunoblot analysis after Triton X-100 subcellular fractionation. Bars represents the F/G actin mean value ± SE of three independent duplicate experiments (*P < 0.05). B) APOPercentage Apoptosis Assay of steroid-stimulated DU145 cells in the presence or absence of androgen receptor antagonist, flutamide (F). Cells pre-treated or not with 10-7 M flutamide (F), were incubated with testosterone-BSA, dihydrotestosterone (DHT), estradiol-BSA (E2-BSA), dexamethasone (DEXA) or control BSA dissolved in serum-supplemented medium at a final concentration of 10-7 M for 24 hours. Cells serum starved for equal period of time served as a positive control for apoptosis. The mean OD measured at 550 nm ± SE of three independent experiments performed in triplicates was normalized to the mean OD ± SE of the untreated control (serum supplemented) cells. Results are presented in bars as percentage (%) of control cells taken as 100% (**P < 0.01).

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Molecular Cancer

ISSN: 1476-4598