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Figure 2 | Molecular Cancer

Figure 2

From: Membrane androgen receptor activation triggers down-regulation of PI-3K/Akt/NF-kappaB activity and induces apoptotic responses via Bad, FasL and caspase-3 in DU145 prostate cancer cells

Figure 2

Long-term dephosphorylation/inactivation of PI-3K in DU145 cells. Serum supplemented DU145 cells were stimulated with 10-7 M testosterone-BSA for the indicated time periods (A: 0, 60 or 120 min and B: 0, 12 or 24 h). Following cell lysis equal amounts of proteins were immunoprecipitated (IP) with an anti-phosphotyrosine (pTyr) antibody. The tyrosine-phosphorylated as well as equal amounts of total lysates were immunoblotted (IB) with a specific antibody against the p85 subunit of PI-3K. The immunoblots were analyzed by densitometry. The intensity of phosphorylated PI-3K bands was normalized to the intensity of the corresponding total PI-3K band. Blots show a representative experiment, while the relative fold decrease in (p85)PI-3K phosphorylation with that of untreated cells taken as 1 are shown in the chart. Each bar represents mean values ± SE of at least three independent experiments (**P < 0.01).

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Molecular Cancer

ISSN: 1476-4598