Skip to main content
Figure 3 | Molecular Cancer

Figure 3

From: Membrane androgen receptor activation triggers down-regulation of PI-3K/Akt/NF-kappaB activity and induces apoptotic responses via Bad, FasL and caspase-3 in DU145 prostate cancer cells

Figure 3

Long-term dephosphorylation/inactivation of Akt kinase in DU145 cells. DU145 cells were exposed to 10-7 M testosterone-BSA for the indicated time periods: (A: 0, 5, 15, 30, 60 and 120 min), (B: 0, 1, 2, 6 h) and (C: 0, 12 and 24 h). The ratio of the cellular content of the phosphorylated (at Ser473 or Thr308 residues) versus the total isoform of Akt kinase was measured in cell lysates by Western blotting using specific antibodies for each form and was normalized to the corresponding ratio of control. Blots show a representative experiment, while the numbers below each lane correspond to the mean values ± SE from three independent experiments (*P < 0.05, **P < 0.01) indicating the fold-decrease in the phosphorylation level for the indicated time point normalized to the corresponding short- and long-term untreated controls, respectively.

Back to article page