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Figure 5 | Molecular Cancer

Figure 5

From: Membrane androgen receptor activation triggers down-regulation of PI-3K/Akt/NF-kappaB activity and induces apoptotic responses via Bad, FasL and caspase-3 in DU145 prostate cancer cells

Figure 5

Testosterone-BSA suppresses the activity of the transcription factor NF-κB, which is reversed by the actin inhibitor cytochalasin B. DU145 cells grown in serum supplemented medium were exposed for 60 min to 10-7 M testosterone-BSA, or to 10-7 M cytochalasin B alone or in combination with testosterone-BSA. TNF-treated HeLa cells or serum supplemented DU145 cells were used as positive controls, 24 h-serum starved DU145 cells as negative control respectively. At the end of the incubation NF-κB activity was measured as described in Materials and Methods. Results are normalized to NF-κB activity in cells cultured in serum-free medium in the absence of steroids and are presented as the mean values ± SE of four independent experiments performed in triplicates (**P < 0.01 for testosterone-BSA treatment versus serum conditions, and #P < 0.01 for testosterone-BSA treatment alone versus testosterone-BSA treatment plus cytochalasin B).

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