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Figure 6 | Molecular Cancer

Figure 6

From: Membrane androgen receptor activation triggers down-regulation of PI-3K/Akt/NF-kappaB activity and induces apoptotic responses via Bad, FasL and caspase-3 in DU145 prostate cancer cells

Figure 6

Testosterone-BSA induces FasL expression, which is inhibited by the actin cytoskeletal drug cytochalasin B and the specific ROCK inhibitor Y-27632. (A) Cells were pre-treated (+) or not (-) with 10-7 M cytochalasin B (cyt B) or Y-27632 (10 μM) for 1 h, and then exposed (+) or not (-) to 10-7 M testosterone-BSA in serum supplemented medium. 24 h serum starved cells served as a positive control for apoptosis. (A) Representative experiment showing FasL expression levels determined by immunoblotting equal amounts of cell lysates with a specific anti-FasL antibody (upper panel). The membrane was then stripped and re-probed with a specific anti-actin antibody to ensure equal protein loading (lower panel). Immunoblots were analyzed by densitometry. The relative fold increase in FasL expression with that of untreated cells taken as 1 is shown in the chart. Each bar represents mean values ± SE of three independent experiments (**P < 0.01).

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