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Figure 2 | Molecular Cancer

Figure 2

From: Inhibition of HIF-1alpha activity by homeodomain-interacting protein kinase-2 correlates with sensitization of chemoresistant cells to undergo apoptosis

Figure 2

HIPK2 inhibited HIF1 transcriptional activity of target genes. (A), H1299 cells were transiently co-transfected with HIPK2-GFP or K221R-GFP expression vectors along with the luciferase reporter gene driven by human erythropoietin enhancer region containing a functional HIF-1 binding site (pEpoE-luc) or a mutant HIF-1 binding site (pEpoEM1-luc). Twelve hours after transfection cells were treated with CoCl2 (200 μM) for 24 h. Thirty-six hours after transfection luciferase activity was assayed. Relative Luciferase Units (RLU) normalized to β-gal is shown. The shown data represent the mean ± SD from three independent experiments performed in duplicate. (B), RKO-C, RKO-p53i, and T98G cells were transfected with HIPK2-GFP or K221R-GFP expression vectors. Twelve hours after transfection cells were treated with CoCl2 (200 μM) for 24 h. Thirty-six hours after transfection cells were harvested and MDR1 and Bcl2 expression was determined by RT-PCR analysis. Aldolase (aldo) was used as loading control. One representative experiment from three independent experiments is shown.

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Molecular Cancer

ISSN: 1476-4598