Figure 6
From: The centrosome protein NEDD1 as a potential pharmacological target to induce cell cycle arrest
Depletion of NEDD1 potentiates the anti-mitotic activity of the Plk1 inhibitor BI2536. (A) Flow-cytometric analysis of HCT116 p53+/+ and p53-/- cell lines treated for 48 h with control or NEDD1-siRNA (0.5 nM) alone or combined with BI2536 (10 nM) and labelled with propidium iodide. (B) Quantification of G2/M HeLa cells following flow-cytometric analysis, after treatment for 48 h with control or NEDD1-siRNA (0.5 nM) alone, or combined with BI2536 (5 nM). (C) Quantification of monopolar spindles (as a percentage of total prometa-metaphase spindles) present in HeLa cells treated for 48 h with control or NEDD1-siRNA (0.5 nM) alone, or combined with BI2536 (5 nM).