Skip to main content
Figure 1 | Molecular Cancer

Figure 1

From: Camptothecin and khat (Catha edulis Forsk.) induced distinct cell death phenotypes involving modulation of c-FLIPL, Mcl-1, procaspase-8 and mitochondrial function in acute myeloid leukemia cell lines

Figure 1

AML cell lines exhibited different sensitivities to khat and CPT. Selected human AML cell lines were exposed to 200 μg/ml khat, 0.1 μM CPT and 1.0 μM CPT for 8 hrs and evaluated for toxic effects. (A) Effects on cell viability/proliferation were assessed using the WST-1 assay. The results were collected by measuring absorbance (450 nm - 620 nm) and are presented as: [(absorbance treated cells)/(absorbance control cells)] × 100. (B) Effects on nuclear morphology were determined following Hoechst staining using epifluorescent microscopy. The results are presented as percentages cells with normal nuclear morphology as compared to controls. The difference in khat sensitivity between MOLM-13 and MV-4-11 was statistical significant as indicated by the asterisk (p < 0.05). The results in A are based on two separate experiments and the results in B based on three separate experiments; all experiments were run in triplicate; the bars represent standard error of mean (SEM).

Back to article page