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Figure 3 | Molecular Cancer

Figure 3

From: Elevated expression of p53 gain-of-function mutation R175H in endometrial cancer cells can increase the invasive phenotypes by activation of the EGFR/PI3K/AKT pathway

Figure 3

p53-R175H induces activation of EGFR/PI3K/AKT pathway. (A) Western blot analysis showing p53 expression in R175H, UT and Vector cells. UT and Vector cells served as the control. The membranes were stripped and probed with anti-pEGFR (pY1173), total EGFR, pAKT (ser473), total AKT antibody, pERK1/2, or total ERK1/2. The membranes were probed with anti-G3PDH antibody to assure even loading of proteins in each lane. (B) Expression of p53-R175H in KLE cells was inhibited by pSUPER-p53 vector (siRNA) as described in Materials and Methods. Cells were harvested and subjected to Western blot to determine mutant p53 expression. UT and Control siRNA cells served as the control. Membranes were stripped and probed with anti-pEGFR (pY1173), total EGFR, pAKT (ser473), or total AKT antibody. G3PDH was determined to ensure even loading of proteins in each lane.

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Molecular Cancer

ISSN: 1476-4598