p53-R175H-induced AKT signaling is mediated by EGFR activation. (A) R175H, UT and Vector cells were seeded in 12-well plates and treated with PD153035 (20 μM). Cell invasion and migration assay were performed at 24 h and 6 h post treatment, respectively. An asterisk indicates P < 0.05. (B) R175H, UT and Vector cells were pretreated with PD153035 (20 μM) for 2 h and harvested for Western blot. Membranes were stripped and probed with anti-pEGFR (pY1173), total EGFR, pAKT (ser473), or total AKT antibody. G3PDH was determined to ensure even loading of proteins in each lane.