Skip to main content
Figure 3 | Molecular Cancer

Figure 3

From: Expression and function of hypoxia inducible factor-1 alpha in human melanoma under non-hypoxic conditions

Figure 3

Quantitative analysis of HIF-1αFL & HIF-1α785 mRNA expression in human melanoma cells. Total RNA was extracted from HEMn-LP, SbCl2, WM1366, and WM9 cells at 72 h (A) after seeding. RNA was then converted to cDNA using the High Capacity cDNA Archive Kit (ABI). Real-Time PCR analysis was performed using TaqMan probes directed at HIF-1αFL or HIF-1α-785 as well as β-actin. The reactions were performed under conditions specified in the ABI TaqMan Gene Quantification assay protocol. Data was corrected for efficiency and loading using the Pfaffl method. Data is expressed as fold change, corrected for β-actin, relative to HeMn-LP. (B) Real-time PCR analysis was performed using Taqman probes directed at HIF-1αFL or HIF-1α785 as well as 18S using RNA extracted from HEMn-LP, WM3211, WM3248, and WM239 cells. Data is representative of at least 2 separate experiments.

Back to article page

Molecular Cancer

ISSN: 1476-4598