Figure 9

Phagocytic and alloantingenic presenting activity of DCs loaded with HT29 iNOS^- cells in the presence of SNP and PTIO. Cells were incubated for 3 h in fresh medium (CTRL), PTIO (100 μmol/L), SNP (100 μmol/L) or both (SNP+PTIO), then stained with PKH2-FITC and co-incubated with DCs for the phagocytosis assay, as described in the Methods section. The dot plot analysis of a phagocytosis assay, representative of three similar experiments performed in duplicate, is shown in panel A. In panel B the phagocytic index is represented as mean ± SE of all the experiments. Versus CTRL: * p < 0.05; versus SNP: ° p < 0.05. C. Unloaded DCs (DC) or DCs loaded with HT29 iNOS^- cells in the same experimental conditions of panel B were inhibited by mitomycin and the DNA synthesis of co-cultured allogenic lymphocytes was measured as reported in the legend of Figure 6. The Proliferation Index was measured as described in the Methods section. The [methyl-³H]deoxythymidine incorporation of lymphocytes alone were 899.33 ± 96.56. The experiments were performed in triplicate and the results are expressed as mean ± SE from four experiments with lymphocytes from different donors. Versus DC: * p < 0.02; versus CTRL: ° p < 0.02; versus SNP: ° p < 0.05.