Table 1 Characterization of HT29, HT29-dx and HT29 iNOS- cells
Parameters | HT29 cells | HT29-dx cells | HT29 iNOS- cells |
|---|---|---|---|
Doxorubicin-induced expression of iNOS protein | Increased | Absent | Absent |
Doxorubicin-induced expression of iNOS mRNA | Increased | Absent | Absent |
Doxorubicin-induced NOS activity | High | Low | Low |
Intracellular doxorubicin Accumulation | High | Low | Low |
LDH release after Doxorubicin | High | Low | Low |
MRP3 expression | Low | High | Low |
Doxorubicin-induced MRP3 nitration | Present | Absent | Absent |
Pgp expression | Low | High | Low |
Doxorubicin-induced Pgp nitration | Absent | Absent | Absent |
Doxorubicin-induced CRT translocation | Increased | Absent | Absent |
Doxorubicin-induced DC activation | Increased | Absent | Absent |