Figure 1
From: Dual effects of TGF-β on ERα-mediated estrogenic transcriptional activity in breast cancer
The Smad3/4 complex confers TGF-β repression on ERα-mediated transcription. Smads (Smad2, Smad3, and Smad4 alone or combinations of Smad2/4 or Smad3/4) or empty vector was co-transfected into MCF-7 cells (a) or MDA-MB-468 cells (b). The cells were treated with (+) or without (-) E2 and/or TGF-β as indicated for 16 hours prior to lysis, and analyzed for luciferase activity. (c-d) MCF-7 cells were transfected with Smad2/4 or Smad3/4 and treated with (+) or without (-) E2 and/or TGF-β as indicated for 8 hours. Total mRNA was isolated and the mRNA levels of PS2 and C-MYC were detected and quantified by reverse transcription and semi-quantitative real time PCR.