Figure 4
From: Dual effects of TGF-β on ERα-mediated estrogenic transcriptional activity in breast cancer
Expression of Smad4 in breast cancer cell lines and ERα-positive infiltrating beast carcinoma. (a and b) MCF-7 and MDA-MB-231 cells were treated with TGF-β for 1 hr and then subjected to immunofluorescence analysis with anti-Smad2/3 and anti-Smad4 antibodies to localize endogenous Smad2/3 and Smad4. Nuclei were visualized by DNA staining with DAPI. (c-g) Immunohistochemical staining of Smad4 in human breast tissue: (c) Benign breast ductal epithelial (1000×). (d) ERα-positive infiltrating breast carcinoma (1000×). (e) Residual benign ductal epithelial (arrow) and surrounding ERα-positive infiltrating breast carcinoma (200×). (f) Residual benign breast ductal epithelial in panel e (1000×). (g) Surrounding infiltrating breast carcinoma in panel e (1000×).