Figure 1

Different GLI1 haplotypes function similarly. A. Different GLI1 haplotypes activate transcription of a CAT reporter comparably in HeLa cells. The GLI1 haplotypes are indicated along the abscissa with the amount transfected (ng) in each experiment. CAT activity, normalized by measuring β-galactosidase activity spectrophotometrically (Promega, Madison, WI), is indicated on the ordinate. Bars represent the means derived from three independent experiments. *indicates p < 0.05 calculated using a test of difference between means, comparing 2798G;3298G with the corresponding amount of each of the other haplotypes. B. Different GLI1 haplotypes transform RK3E cells comparably. The p value represents a test of difference between means, comparing 2798G;3298G with each of the other GLI1 haplotypes.