Figure 1From: An MDM2 antagonist (MI-319) restores p53 functions and increases the life span of orally treated follicular lymphoma bearing animalsChemical structure of MI-319 and MI-219 and MDM2 protein binding assay. (A), Chemical structure of MI-319 and MI-219. (B), Fluorescence polarization-based MDM2 binding assay. The binding affinities (Ki values) were determined by a competitive fluorescence polarization-based binding assay using recombinant His-tagged MDM2 (amino acids 1-118) and PMDM6-F (5-FAM-βAla-βAla-Phe-Met-Aib-pTyr-(6-Cl-L-Trp)-Glu-Ac3c-Leu-Asn-NH2), a fluorescently labeled high-affinity p53-based peptide.Back to article page