Identification and isolation of GM3 and iGb3 fromF3A and F4A. A) Fractions 20 μg, GM3 5 μg and iGb3 5 μg were chromatographed and stained with orcinol. The region corresponding to Gb3/iGb3 in an F3A preparative HPTLC was scraped off and extracted with C-M (1:1). The concentrated extract was examined by HPTLC and revealed with orcinol (iF3A). B) The same procedure as in (A) was used to examine F4A and generate iF4A. Arrows indicate bands with RF corresponding to GM3 (double band) in F3A and F4A; * the same for Gb3/iGb3.