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Figure 6 | Molecular Cancer

Figure 6

From: Identification of iGb3 and iGb4 in melanoma B16F10-Nex2 cells and the iNKT cell-mediated antitumor effect of dendritic cells primed with iGb3

Figure 6

ESI-LIT-MS analysis of GM3-depleted permethylated neutral glycolipids of F3A fraction. A) Total ion-mapping of m/z 667, marker of Gb3 and iGb3. B) MS4 spectrum of the daughter-ion m/z 445 obtained after MS3 fragmentation (not shown) of the parent-ion at m/z 1215 observed in A. The fragments at m/z 371 (3.4%) and m/z 211(1.3%) are typical of iGb3, whereas the fragment at m/z 329 (100%) is a marker of Gb3. C) Total ion-mapping (TIM) of m/z 912, marker of Gb4 and iGb4. D) MS4 spectrum of the daughter-ion m/z 431 obtained after MS3 fragmentation (not shown) of the parent-ion at m/z 1460 observed in B . The fragments at m/z 329 (2.5%), m/z 357 (1.4%), and m/z 369 (2.7%) are characteristic of iGb4, whereas the fragment at m/z 315 (100%) is a marker of Gb4. m/z, mass to charge ratio.

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