Figure 5

ELISA test for MMP2 and VEGF quantification on osteosarcoma cell line supernatants. The concentration of secreted MMP2 (a) and VEGF (B) on 48 h conditioned supernatants of untreated (Black bars) and 5 μM sorafenib treated (white bars) OS cells was shown per 106 cells as Mean +/- standard deviation (Y error bars) of 2 different experiments in triplicate. a, p < 0.05 versus untreated cells; b, p < 0.05 versus sorafenib.