Table 1 Sequences (5'- 3') of primers and probes used in quantitative real time RT-PCR analysis of mouse (m) and human (h) gene expression.
From: Paracrine sonic hedgehog signalling by prostate cancer cells induces osteoblast differentiation
Gene | Forward Primer | Probe | Reverse Primer |
|---|---|---|---|
mGli1 | GGAAGTCCTATTCACGCCTTGA | TCAAGACGCACCTTCGGTCGCAC | CAACCTTCTTGCTCACACATGTAAG |
mPtc1 | CTCCAAGTGTCGTCCGGTTT | CGTGCCTCCTGGTCACACGAACAA | ACACCGTGGTCTGAGAGCTGTAC |
mAkp2 | TCAGGGCAATGAGGTCACATC | CGCTGGGCCAAGGATGCTGG | CACCCGAGTGGTAGTCACAATG |
mRunx2 | CGAAATGCCTCCGCTGTTAT | TAGCCAGGTTCAACGAT | CGCTCCGGCCCACAA |
mOcn | GGCCCTGAGTCTGACAAAGC | TTCATGTCCAAGCAGGAGGGCA | GCCGGAGTCTGTTCACTACCTT |
mOpn | CATGAAGAGCGGTGAGTCTAAGG | TCCCTCGATGTCATCCCTGTTGCC | CTTTCCGTTGTTGTCCTGATCA |
mGapdh | AACCTGCCAAGTATGATGACATCA | TGAAGCAGGCATCTGAGGGCCC | CTGTTGAAGTCGCAGGAGACAA |
hSHH | AAGGACAAGTTGAACGCTTTGG | ATCTCGGTGATGAACCAGTGGCCAG | TCGGTCACCCGCAGTTTC |
hGLI1 | GGGCACCATCCATTTCTACAGT | AGCCCAAGAGGGAGCGGGAAGG | TCAGTCTGCTTTCCTCCCTGAT |
hGAPDH | CGACAGTCAGCC GCATCTT | CGCCAGCCGAGCCACATCG | TGACCAGGCGCCCAATAC |