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Figure 4 | Molecular Cancer

Figure 4

From: Lack of functional and expression homology between human and mouse aldo-keto reductase 1C enzymes: implications for modelling human cancers

Figure 4

Reduction of 5α-DHT to 3α/β-adiol by human and murine AKR1C enzymes. Human and murine recombinant AKR1C enzymes were incubated with 25 μM 5α-DHT mixed with 1 μCi [3H]-5α-DHT and NADPH. At 30 and 90 minutes reactions were stopped and the steroids extracted and separated in TLC along with known standards. The radioactive traces were scanned and the percentage of 5α-DHT reduced to 3α- or 3β-adiol calculated. Bars represent average expression of three replicates and error bars represent standard deviation. * - Incubation with AKR1C21 resulted in efficient conversion to a product other than 3α- or 3β-adiol.

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