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Figure 1 | Molecular Cancer

Figure 1

From: 2-Deoxy-D-glucose enhances TRAIL-induced apoptosis in human melanoma cells through XBP-1-mediated up-regulation of TRAIL-R2

Figure 1

2-DG sensitizes melanoma cells to TRAIL-induced apoptosis. A, 2-DG inhibits melanoma cell growth. Upper panel: Mel-RM and MM200 cells were treated with 2-DG at indicated concentrations for 48 hours. Cell viability was measured by MTT assays. Lower panel: Mel-RM and MM200 cells treated with 2-DG (10 μM) for indicated periods were subjected to measurement of viability using MTT assays. B, Mel-RM (upper panel) and MM200 (lower panel) cells were co-treated with 2-DG (10 μM) and TRAIL (200 ng/ml) for indicated periods before apoptosis was measured by the propidium iodide method using flow cytometry. C, Representative flow cytometry histograms of apoptosis assays. Mel-RM and MM200 cells were treated with 2-DG (10 μM), TRAIL (200 ng/ml), or the combination of both for 24 hours. Apoptosis was measured by the propidium iodide method using flow cytometry. D, Whole cell lysates from Mel-RM and MM200 cells treated with the combination of 2-DG (10 μM) and TRAIL (200 ng/ml) for 16 hours were subjected to Western blot analysis. Note that PARP was detected by an Ab that specifically recognizes the cleaved form of PARP.

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