All the SHH signaling pathway components are expressed in human CRCC tumors. (A) Western blot analysis of the SHH ligand in 9 human tumors (T1 ...) and normal corresponding tissues (N1 ...) lysates incubated with antibodies against human SHH ligand and corresponding β-actin. The gels shown are representative for at least 3 independent experiments. (B) Quantitative gene expression of Ptch1 and Smo receptors, and of the Gli1 transcription factor in the same normal/tumoral tissue pairs shown in (A). Results are representative of 4 independent experiments.