Figure 3From: The sonic hedgehog signaling pathway is reactivated in human renal cell carcinoma and plays orchestral role in tumor growthThe inhibition of the SHH signaling pathway decreases human CRCC cell proliferation. (A) Human 786-0 cells were treated in control (Ctl) or with cyclopamine (Cyclopamine, 20 μM) and cells were counted each day. Results are shown as mean ± SEM, n = 6; *, P < 0.05; **, P < 0.01 from Ctl. (B) Human 786-0 cells were treated for 5 days in control (Ctl) or with cyclopamine (Cyclopamine) and adherent cells were counted. Results are shown as mean ± SEM, n = 6; **, P < 0.01 from 0 (without cyclopamine). (C) Our panel of human CRCC cells either deficient or expressing VHL were treated treated for 5 days in control (Ctl) or with cyclopamine (Cyclopamine, 20 μM) and adherent cells were counted. Results are shown as mean ± SEM, n = 6; **, P < 0.01 from Ctl (without cyclopamine) that was set to 100%. (D) Human 786-0 wt, 786-0 V, 786-0 VHL and 786-0 πVHL cells were analyzed for BrdU incorporation after treatment in control (Ctl) or with cyclopamine (Cyclopamine, 20 μM), for the indicated periods of time. Results are shown as mean ± SEM, n = 6; *, P < 0.05; **, P < 0.01 from corresponding Ctl.Back to article page