Figure 3

The inhibition of the SHH signaling pathway decreases human CRCC cell proliferation. (A) Human 786-0 cells were treated in control (Ctl) or with cyclopamine (Cyclopamine, 20 μM) and cells were counted each day. Results are shown as mean ± SEM, n = 6; *, P < 0.05; **, P < 0.01 from Ctl. (B) Human 786-0 cells were treated for 5 days in control (Ctl) or with cyclopamine (Cyclopamine) and adherent cells were counted. Results are shown as mean ± SEM, n = 6; **, P < 0.01 from 0 (without cyclopamine). (C) Our panel of human CRCC cells either deficient or expressing VHL were treated treated for 5 days in control (Ctl) or with cyclopamine (Cyclopamine, 20 μM) and adherent cells were counted. Results are shown as mean ± SEM, n = 6; **, P < 0.01 from Ctl (without cyclopamine) that was set to 100%. (D) Human 786-0 wt, 786-0 V, 786-0 VHL and 786-0 πVHL cells were analyzed for BrdU incorporation after treatment in control (Ctl) or with cyclopamine (Cyclopamine, 20 μM), for the indicated periods of time. Results are shown as mean ± SEM, n = 6; *, P < 0.05; **, P < 0.01 from corresponding Ctl.