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Figure 5 | Molecular Cancer

Figure 5

From: The sonic hedgehog signaling pathway is reactivated in human renal cell carcinoma and plays orchestral role in tumor growth

Figure 5

Cyclopamine treatment decreases the expression of the SHH ligand, Smo receptor, Gli1 factors, and increases the expression of the Ptch1. (A) Human 786-0 CRCC cells were seeded and treated for the indicated periods of time in control (Ctl) or with cyclopamine (Cyclopamine, 20 μM). Shown are western blot analysis of the SHH ligand in cell lysates from cells treated in each condition and incubated with antibodies against human SHH ligand and corresponding β-actin. The gels shown are representative for at least 3 independent experiments. (B) Human 786-0 CRCC cells were seeded and treated for the indicated periods of time in control (Ctl) or with cyclopamine (Cyclopamine, 20 μM). Quantitative gene expression of Ptch1, Smo receptors, Gli1, Gli2 and Gli3. Results are shown as mean ± SEM, n = 6 *, P < 0.05 from cells treated in Ctl at the same time point.

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Molecular Cancer

ISSN: 1476-4598