Skip to main content

Advertisement

Figure 6 | Molecular Cancer

Figure 6

From: The sonic hedgehog signaling pathway is reactivated in human renal cell carcinoma and plays orchestral role in tumor growth

Figure 6

Inhibition of the SHH signaling pathway induces tumor regression in nude mice. (A) Tumor growth in mice treated according to the first experimental protocol. Results are shown as mean ± SEM, n = 7 for both groups; **, P < 0.01 cyclopamine-treated mice vs Ctl-treated mice. Immunoblotting experiments to measure expression of Gli1 in tumors lysates. The gels shown are representative for at least 3 independent experiments. Photographs show the implanted tumors in representative mice at day 1 of drug injection, at day 19 in Ctl-treated group or cyclopamine-treated group. (B) Tumor growth in mice treated according to the second protocol. Results are shown as mean ± SEM, n = 7 for both groups; **, P < 0.01 cyclopamine-treated mice vs Ctl-treated mice. (C) Left, tumor sections of control- (Ctl) or cyclopamine (Cyclopamine)-treated mice immunostained with an antibody against Ki67 (magnification ×400). Right, proliferative index. Results are shown as mean ± SEM, n = 7. **, P < 0.01 from Ctl-treated mice. (D) Left, tumor sections of control- (Ctl) or cyclopamine (Cyclopamine)-treated mice immunostained for CD31 (magnification ×400). Right, quantification of neovascularization. Results are shown as mean ± SEM, n = 7. *, P < 0.05 from Ctl-treated mice.

Back to article page