Effects of LN on Gem-induced apoptosis in AsPC-1 cells. AsPC-1 cells were treated as described in results. After Gem treatment for 72 h, cell apoptosis was examined by Hoechst staining (the arrow indicates the apoptotic cells) (A), flow cytometry analysis of Annexin-V labeling (B) and western blot analysis of cleaved caspase-3 protein expression (C). Bars represent the mean of three independent experiments ± SE. *, P < 0.05, vs. cells on plastic without Gem treatment; #, P < 0.05, vs. cells on plastic treated with Gem.