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Figure 3 | Molecular Cancer

Figure 3

From: CXCR4 expression heterogeneity in neuroblastoma cells due to ligand-independent regulation

Figure 3

Effect of Cell Density on CXCR4 Surface Expression. Individual flasks of culture media were each synchronously seeded with 5 × 106 SK-N-SH cells and grown at 37°C to varying confluency. At each confluency, cells were harvested and processed for cell surface staining of CXCR4. The maximum CXCR4 signal was observed at 50% confluency and set as 100% mean fluorescent intensity. All intensities are represented as % relative values normalized to the maximum intensity. Result represents the mean from three experiments and shows the correlation between cell density and surface expression (Pearson correlation, R2 = 0.9488, P < 0.05).

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