Activation of the Notch pathway during breast cancer progression. (A) Photomicrographs represent staining of normal, hyperplastic, DCIS, and breast cancer tissue sections with antibodies that specifically recognize the cleaved and active form of Notch1, and Hes5; magnification 20×. Insets show nuclear staining at higher magnification (40×). (B) Photomicrographs represent staining of normal and cancerous breast tissue with antibodies against Numb, Hes5 and Cleaved Notch1; magnification 20×. (C) Scatter plot represents expression of cleaved Notch1, Hes1/5, and Numb across various normal (blue) and cancer (red) breast tissues analyzed. The total number of cases analyzed under each category (n) is mentioned below each column, and the black bar represents the median score.